| 학회 |
한국고분자학회 |
| 학술대회 |
2015년 봄 (04/08 ~ 04/10, 대전컨벤션센터) |
| 권호 |
40권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Detection of SK-BR-3 Cells using Herceptin-conjugated Liquid Crystal Microdroplets as a Hosting and Sensing Platform |
| 초록 |
In this study, we report an recognition in which antibodies are conjugated on the surface of liquid crystal (LC) droplets rather than on solid surfaces. 4-Cyano-4´-pentylbiphenyl(5CB, nematic liquid crystal) microdroplets were prepared by using O/W emulsion system. Amphiphilic diblock copolymer of poly (styrene)-b-poly (acrylic acid) (PS-b-PA) was used as a surfactant to prepare the emulsion. The hydrophobic styrene block is miscible with the hydrophobic 5CB and incorporates in the micelle core. The hydrophilic PA block is in the outer shell. Herceptin was subsequently conjugated to the PA block to preferentially target SK-BR-3 cells (human breast cancer cells) that have HER2 receptors on the cell membrane. The configuration of the LC (5CB) in the emulsion was changed as a result of antigen-antibody interaction forces. This observation thus demonstrates potentiality of the antibody-conjugated LC emulsions as a biosensor for specific cell and protein detection. |
| 저자 |
왕딩, 강인규
|
| 소속 |
경북대 |
| 키워드 |
Liquid crystal microdroplets; copolymer; antibody
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| E-Mail |
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