| 학회 |
한국고분자학회 |
| 학술대회 |
2019년 봄 (04/10 ~ 04/12, 부산컨벤션센터(BEXCO)) |
| 권호 |
44권 1호 |
| 발표분야 |
고분자가공/복합재료 |
| 제목 |
Matrix Metalloproteinase (MMP)-responsive gold nanoparticles (AuNP) to form ‘Clicked’ Nanoclusters |
| 초록 |
To overcome MMP responsive assembly of AuNP, we surface-decorated AuNP both with azide-functionalized PEG(N3-PEG) and PEG chain with MMP-cleavable-peptide(MMP CP), which can be clicked into clusters in response to MMP. To synthesize nanoparticle, we sequentially conjugated methoxy-PEG(mPEG) to MMP CP and then conjugated mPEG-MMP CP to the AuNP co-decorated with activated PEG and N3-PEG. Azide moieties are shielded in PEG shells and those are only exposed when PEG shells are deshelled by MMP, which can be assembled into nanoclusters by click reaction. We characterized the size of the nanoparticle by DLS, where the particles was increased from 34nm to 64nm after PEG decoration. We additionally characterized the PEG shell of nanoparticle by TEM, where the thickness of PEG shell was shown to be 2nm. When Alexa647-alkyne was added to the AuNP, fluorescence tagging did not occur, but when the PEG shells was peeled off by MMP, we observed increased fluorescence from the AuNP. |
| 저자 |
신지운, 유혁상, 손영주, maowei, 이주원, 박재근
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| 소속 |
강원대 |
| 키워드 |
gold nanoparticle; Matrix Metalloproteinase; click reaction
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| E-Mail |
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