| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.455 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Adhesion and growth of human umbilical vein endothelial cells on collagen-treated PU/PEGDA IPNs |
| 초록 | Biohybridization between cell and polymer becomes common tool to construct artificial organs as a long-term prosthesis. There are two kinds of the biohybrid systems. One is to biohybridize the target cell on the biocompatible and non-degradable polymer surface, and the other is to biohybridize the target cell on the biodegradable polymer scaffold. In the latter system, the polymer is totally substituted by the tissue composed of the grown cells. The biological stability of the cells and tissue is much better in the case of the latter system, compared to that of the first system. Because natural blood vessels are lined by an endothelial cell monolayer, which are the ideal nonthrombogenic surface under normal physiologic condition, biohybridization between ECs and biodegradable amphiphilic IPNs was proposed. Endothelialization is one of the methods to enhance blood compatibility. So many efforts have been made to mimic this natural vascular system and introduce the endothelial lining to the inner wall of artificial vascular vessel. Otherwise the adhesion and proliferation of EC on the synthetic biomaterials are definitely affected by the surface group, surface free energy, surface charge density of the biomaterials, so there has been considerable progress with respect to improving EC attachment and growth through the surface modifications considering the EC-biomaterial interactions. One of the methods to enhance the adhesion of EC on biomaterials is the collagen treatment on the surface of biomaterial. In our study, amphiphilic PU/PEGDA IPNs was synthesized with different surface energy to investigate the effect of adhesion and growth of human umbilical endothelial cells(HUVECs). To enhance the adhesion of HUVECs, collagen having cell-binding molecules was adsorbed on the surface of PU/PEGDA IPNs. All IPNs had micro-separated sea-island morphology. The dispersed PU-rich phase domain size decreased with increased crosslinking density. The morphology of the collagens adsorbed on the IPN surfaces highly depended on the surface energy of the IPNs. As the surface becomes hydrophilic, the adsorbed collagens on IPN surface aggregated more. Because the binding energy of the collagens on the relatively hydrophilic surface of the IPN 4k, 6k was weak, the adsorbing collagens aggregated easily compared to IPN 2k. Human umbilical vein endothelial cells(ECs) were successfully adhered onto the collagen-immobilized IPN surface, and the maximum value of the ECs-adhesion was similar with that of TCPS. After 1 or 3 hour-incubation, the adhesion rate of collagen-treated IPN was higher than that of non-treated IPN. The morphology of adhered ECs on IPN 2k-C and IPN 2k after 1-day and 3-day incubation showed that ECs adhered on IPN surface spread successfully. The adhesion and the spreding increased on the non-treated IPN surfaces by increasing of the hydrophobicity of the IPNs. IPN 2k-C has the proper surface energy for adhesion and proliferation of ECs. Therefore the platelet adhesion was significantly reduced on the EC-hybridized surface of the IPNs. |
| 저자 | 윤성수, 윤준진, 박태관, 김성철, 윤남균 |
| 소속 | 한국과학기술원 기능성고분자신소재연구센터 |
| 키워드 | endothelial cell; biodegradabale IPN; surface energy; collagen treatment |
