| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 고분자 가공/블렌드 |
| 제목 | Preparation of Silk Sericin Beads and its Application as an Immobilization Support of Enzymes |
| 초록 | Silk protein from Bombyx mori. consist of two proteins, fibroin and sericin. Fibroin is the main component of silk protein and it is the protein that we use as a fiber. Sericin possesses only 25% of total silk protein and it envelops two strands of fibroin fiber to hold them together. In general, this protein is removed by the process so called “degumming process”. The luster and touch of silk fiber is gained after the degumming process. The removed sericin was usually treated as waste. Recently, there were many efforts to reuse sericin as biopolymers. However, the concentration of sericin solution should be low (approx. 1%wt) to avoid the gelation of sericin. This limits the fabrication method of sericin where the viscosity of solution became a critical factor. In this study, this problem has been solved by using LiCl/DMSO solvent system. Up to 30% (w/v) of sericin solution could be prepared by this method. The sericin solution was dropt into methanol and various shapes, such as rings, dishes and beads, are formed according to the dropping height and the concentration of sericin solution. At low concentrations (less than 15%), rings are formed, whereas dishes and beads are formed at high concentrations. Appropriate height was choosen in order to prepare sericin beads. The strength of beads increased with the increase of sericin concentration. These beads were used as an immobilization support of enzymes. The enzymes were immobilized onto the surface of sericin beads using glutaraldehyde. During this step, the surface of sericin beads was also cross-linked and the mechanical properties of beads enhanced remarkably. The activity of immobilized enzymes was increased compared to the former results, and also, it had excellent stability against organic solvent. It is thought that the high portion of serine in sericin might be playing a role in these results. Figure. SEM image of sericin bead |
| 저자 | 이기훈 |
| 소속 | 서울대 |
| 키워드 | Silk sericin; Bead; Enzyme Immobilization |
