| 초록 |
The Fe3O4 nanoperticles were prepared by coprecipitating Fe2+, Fe3+ and oxalate in an ammonia solution, and the next step was the binding of surfactant with nanoparticles. Surfactant bound Fe3O4 nanoparticles were characterized with Fourier transform infrared spectroscopy (FTIR), Transmissiion electron microscope (TEM), X-Ray difraction methods. Fe3O4 nanoparticles showed superparamagnetic property. The analysis of Fourier transform infrared spectroscopy confirmed the binding of lipase onto magnetic nanoparticles. The activity of the immobilized lipase for the hydrolysis of olive oil was observed, indicating the lipase bound nanoparticles exhibited higher enzymatic activity than that of crude lipase. Moreover, the immobilized lipase could be reused for several times after recovered by magnetic separations. |
