| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.1584 |
| 발표분야 |
생물화공 |
| 제목 |
Comparing Escherichia coli B and K strains in metabolic engineering |
| 초록 |
Butyric acid pathway genes were introduced to Escherichia coli from Clostridium acetobutyricum strain. The butyric acid synthetic genes consisting of thiL and HCB operon (hbd, crt, bcd, and etfA/etfB) were cloned and expressed under T7 promoter in E.coli. Acyl-CoA thioesterase II (tesB) gene from E.coli MG1655 was also overexpressed. These genes were overexpressed in both E.coli BL21(DE3) and JM109(DE3). The concentration of 0.3 g/L butyric acid was achieved from shake flask culture containing LB fed with 1% glycerol in both strains. To improve the productivity, we deleted pta (phosphate acetyltransferase) and ptsG (a glucose-specific PTS enzyme) in both E. coli strains. The deletion mutants produced more butyric acid under a similar fermentation condition. The effects of metabolic gene knock-outs and amplifications were compared in the two E. coli strains. |
| 저자 |
정세미1, 정무영1, Nagendra Kurumbang1, 상병인2, 오민규1
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| 소속 |
1고려대, 2한국과학기술(연) |
| 키워드 |
Escherichia coli; Clostridium acetobutyricum; butyric acid;
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| E-Mail |
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| 원문파일 |
초록 보기 |
