| 학회 |
한국재료학회 |
| 학술대회 |
2018년 가을 (11/07 ~ 11/09, 여수 디오션리조트) |
| 권호 |
24권 2호 |
| 발표분야 |
B. 나노화학/바이오 재료 분과 |
| 제목 |
Characterization and evaluation of periodic heat shock on MC3T3E1 pre-osteoblastic cells loading 3D Biphasic Calcium Phosphate ceramic scaffold during osteogenic differentiation. |
| 초록 |
Bone diseases like osteoporosis, osteoarthritis etc. can be healed by elevating temperature of bone forming cells and it can also eliminate the risk of bone surgery option like bone grafting. In this study, thermal effects on MC3T3-E1 pre-osteoblastic cell differentiation in biphasic calcium phosphate scaffold were investigated. Cells were given mild heat shock repeatedly during differentiation into osteoblasts cell on BCP-sponge scaffold. The objective of this work is to find out the repeated heat shock on MC3T3E1 cell during differentiation and the differentiation is compared between heated and non-heated cells. We hypothesized that our heat fluctuation on osteogenic differentiation cells were significantly increased their numbers to facilitate the bone formation. After cell seeding on scaffold, they were divided into four different samples based on heating time. Heat shock at 41°C for 1h will be given to Cell loaded scaffolds after 24h, 12h and 6h interval to different samples respectively through 7 day and the effect will be observed. During interval all samples kept in 370c. Cellular viability, morphology and protein expression were investigated after heat-shock. Cell morphology and viability were observed by F-actin staining and MTT assay respectively. The analysis of induction of osteogenic differentiation and the extent of mineralization was also performed using quantitative ALP and calcium assays, respectively. The gene expression of the osteo-specific markers osteopontin (OPN), osteocalcin, collagen type I and the transcription factor Runx-2 were measured by real-time reverse transcription polymerase chain reaction (PCR) and heat shock proteins (HSP70) expression will be further determined by Western blot analysis. Therefore, our study revealed that the periodic heat treatment may effect on osteogenic differentiation potentially and improve bone formation. |
| 저자 |
Shithima Sayed1, Byong-Taek Lee2
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| 소속 |
1Department of Regenerative Medicine, 2Colleage of Medicine Soonchunhyang Univ. |
| 키워드 |
heat shock; BCP; pre-osteoblstic cell; heat shock protein
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| E-Mail |
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