| 초록 |
Cancer immunotherapy has recently been successful in the treatment of various types of tumors. Cytotoxic lymphocytes, including cytotoxic T lymphocytes (CTLs), natural killer (NK) cells, play an essential role in elimination of tumors by directly killing tumor cells. Therefore, evaluation of lymphocyte cytotoxicity against tumor cells is critical for the improvement of cancer immunotherapy. Lymphocyte cytotoxicity is a strictly regulated function requiring a multi-step “checkpoint” to minimize normal cell damage. However, current cytotoxicity assays mostly provide information about final outcomes of cytotoxicity. To overcome this limitation, we are developing new assays that allow “stepwise” evaluation of lymphocyte cytotoxicity using dynamic imaging and microfabrication techniques. |
