| 초록 |
Bio-based production of 1,5-diaminopentane, also known as cadaverine, from renewable feedstock is a promising and sustainable alternative to the petroleum-based production. Here, we report development of a metabolically engineered Corynebacterium glutamicum strain that produces it. L-lysine decarboxylase, which converts L-lysine directly to 1,5-diaminopentane, was amplified by plasmid-based overexpression of the cadA gene under the tac promoter in an industrial L-lysine producer C. glutamicum (U2 strain). However, the recombinant C. glutamicum (U2/pCEcadA) did not produce 1,5-diaminopentane and L-lysine was still detected in the culture medium. Thus, the cadA gene was modified using a codon adaptation program. With the redesigned cadA gene, we achieved 31.94 g/L of 1,5-diaminopentane from glucose without exogenous feeding of L-lysine by fed-batch fermentation of C. glutamicum (U2/pCEcadA). |
