| 초록 |
Bone marrow-derived mesenchymal stromal cells (BMSCs) have a great potential to differentiate into multiple lineages of specialized cells. In this study, a biomimetic ECM network that is derived from target tissue cells is created. We hypothesize that this self-assembled ECM-based structure may provide stem cells with a microenvironment for cell adhesion and even differentiation. A porous composite scaffold was fabricated using a mixture of PLGA, hydroxyapatite and β-tricalcium phosphate. NIH3T3 and MC3T3-E1 were then loaded onto the scaffolds and cultured and the cell-scaffold constructs were subjected to decellularization. After the characterization of the decellularized scaffolds (DCS), rabbit BMSCs were seeded into the DCS. The MSC-DCS constructs were cultured in a osteogenic medium for 2 and 4 weeks, respectively. Mineralizaiton and Calcium deposition were confirmed in Von kossa and alizarin red S staining. |
