| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-정밀화학 |
| 제목 |
Label free analysis of multivalent ion binding using bioresponsive hydrogels and SPR |
| 초록 |
Metal ions play important regulatory roles in the cellular and subcellular systems. For instance, divalent ions such as magnesium and calcium are known to regulate enzymes for various chemical reactions. Hence, accurate determination of metal ion binding events is required for better understanding of the enzymatic reactions in the biological system. Here, we report a method to distinguish between monovalent and multivalent metal ion binding. With the use of a hydrogel modified SPR chip, we were able to show that in comparison to monovalent ions, multivalent ions in the +2 and +3 oxidation states cause a greater extent of amplification in SPR signals. Hence, our results suggest that the evaluation of the oxidation state of metal ions is possible through SPR analysis. |
| 저자 |
Teoh Jie Ying, 양해민, 유동원, 임국희, 전수환
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| 소속 |
서울대 |
| 키워드 |
hydrogels; ion binding; label free; surface plasmon resonance; biosensor
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| E-Mail |
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