| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 가을 (10/23 ~ 10/25, 대전컨벤션센터) |
| 권호 |
25권 2호, p.1470 |
| 발표분야 |
생물화공 (Biochemical Engineering) |
| 제목 |
Microfluidic based single cell analysis of signaling crosstalk between cell wall integrity and mating pathway in S. cerevisiae |
| 초록 |
Here, we investigate the signaling cross-talk between cell wall integrity (CWI) and mating pathway of budding yeast via microfluidic device and quantitative single cell analysis. The microfluidic device enables to 1) track single yeast cells response in continuous media flow with well-focused microscopic images by holding the cells underneath of PDMS micropad, and 2) modulate magnitude of a cell wall stress by dilution of hydrogen peroxide in constant concentration of mating factor. Firstly, we monitor the expression of a mating-specific reporter, based on the FIG1 (Factor Induced Gene 1) promoter driving the expression of quadruple-Venus fluorescent protein. As a result, we found the activation of CWI signalling inhibits mating signaling, which is shown as decrease of Fig1 expression upon increase of cell wall stress magnitude. We expect this simple microfluidic-based single cell analysis will allow us to study a wide range of mutants and explore further the cross-talk mechanisms used by the cells to adapt and distinguish between multiple stresses. |
| 저자 |
이병진1, 정성근1, Sung Sik Lee2, Matthias Peter2, 이창수1
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| 소속 |
1충남대, 2ETH Zurich |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
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