| 학회 |
한국고분자학회 |
| 학술대회 |
2017년 봄 (04/05 ~ 04/07, 대전컨벤션센터) |
| 권호 |
42권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
BMP-2 linked thermo-sensitive hydrogel for bone tissue engineering |
| 초록 |
Lately, bone morphogenetic proteins (BMP) are used for bone treatments. However, the amounts of BMP for treatments is much higher than physiological concentration, because of short half-life and instability of the BMP. In this work, we synthesized the MPEG-b-(ε-caprolactone–ran-α-chloro-ε-caprolactone) (MC-Cl), and then chlorides of the MC-Cl are modified to azides (MC-N3). The BMP-2 containing alkyne group was introduced MC-Cl by cycloaddition. BMP-2 linked MC-N3 (MC-BMP2) was dispersed at distilled water. MC-BMP2 solution was blended with human periodontal ligament stem cells (hPDLSC) and implanted to dorsum of nude mouse. Injected the solution immediately formed a gel-state. hPDLSC in MC-BMP2 hydrogel were proliferated and differentiated to osteoblast like cells. We observed osteoblastic phenotypes and gene expression through histological methods and qRT-PCR respectively. In conclusion, we confirmed that MC-BMP2 hydrogels could increase a half-life and a stability of the BMP-2. |
| 저자 |
박승훈, 박지훈, 이보근, 허지연, 김민주, 김문석
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| 소속 |
아주대 |
| 키워드 |
hydrogel; BMP-2; osteogenic differentiation; human periodontal ligament stem cell
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| E-Mail |
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