| 초록 |
Non-viral vectors have become a popular research topic in the field of gene therapy. In this study, we conjugated short oligopeptides to PAMAM G4to achieve higher transfection efficiency. Previous reports have shown that the PAMAM G4-histidine-arginine dendrimer enhances gene delivery by improving cell penetration and internalization mechanisms. Therefore, we synthesized PAMAM G4-HFR, PAMAM G4-FHR and PAMAM G4-FR derivatives to determine the best gene carrier with the lowest toxicity. Physicochemical studies were performed to determine mean diameters and surface charge of PAMAM derivatives/pDNA polyplexes. DNA condensation was confirmed using a gel retardation assay. Cytotoxicity and transfection efficiency were analyzed using HeLa and HepG2 cells. Therefore, our results show that these carriers are promising and may help achieve higher transfection with negligible cytotoxicity. |
