| 초록 |
Herein, we present a pH-responsive dynamic DNA nanocluster based on gold nanoparticles with highly packed nucleic acid assembly and evaluate its potential as a drug delivery carrier with tumor-specific accumulation. Each gold nanoparticle was readily functionalized with various DNA sequences; indeed, we modified the surface of the nanoparticles with bcl-2 antisense and i-motif binding sequences. Clustering of nanoparticles induced by hybridization of each DNA via i-motif sequence provided tumor targeting and drug loading capabilities. After cellular uptake, the drug was released by disassembly of the cluster into single nanoparticles in response to pH decrease in the late endosome. Furthermore, the antiapoptotic Bcl-2 protein was down-regulated by the antisense-modified gold nanoparticles; thus, drug-mediated apoptosis was further accelerated by sensitizing the cancer cells to the drug. Our size-tunable clustered nanoparticles provide tumor-homing in the blood circulation and are thus a potential multifunctional therapeutic agent in vivo as well as in vitro. |
