| 초록 |
Hepatitis B virus (HBV) infection is a global health issue causing severe liver diseases, such as liver cirrhosis and hepatocellular carcinoma. Monitoring of the serum hepatitis B surface antigen (HBsAg) level is important to the diagnosis of HBV infection for determining whether the disease is acute or chronic, and for estimating the prognosis of chronic hepatitis. In this study, we demonstrate liquid-ion gated field-effect transistor type HBsAg aptasensor, composed of aptamer conjugated carboxylated polypyrrole nanowires (CPNWs) fabricated by electropolymerization and acid treatment. The 3D structure of CPNWs with high length-to-diameter (L/D) ratio and sufficient surface modification enhanced the loading amount of aptamers. As a result, the aptasensor showed high sensitivity toward HBsAg, with low detection limit of 1 fM. Furthermore, this aptasensor could distinguish HBsAg from human saliva, showing a potential for the noninvasive diagnose of HBV infection. |
