| 초록 |
We investigated the in vitro three dimensional vascularized skin on a chip to mimic the structures and functional responses of the human skin using collagen/fibrin hydrogel(CFH) for dermis and poly(l-lactide-co-caprolactone)(PLCL) nanofibrous electrospun membrane(NEM) for epidermis. To mimic vessels, we created channels in the CFH and coated with human umbilical vein endothelial cells(HUVECs). Consequently, we could find that channels coated with HUVECs formed a vessels-like structure, and microvascular networks were formed in CFH like the dermis. Moreover, it was confirmed that PLCL-NEM formed the epidermis like structure. Our results suggest that the vascularized skin-on-a-chip model can potentially be used for testing the toxicity of cosmetics or drugs by three-dimensionally simulating the native human skin structure. |
