| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생체재료 |
| 제목 |
A Novel pH-Responsive Polymer Based Enzyme Free Stem Cell Detachment System for Skin Regeneration |
| 초록 |
Stem cell injection to skin wound site is a promising approach for wound healing. Trypsin treatment for detaching cells accompanies loss of extracellular matrix and often cause cell damage. In this study we synthesized novel pH-responsive polymer based on poly(amino ester) (mPAE) which can provide the appropriate cell culture condition for cell growth without cytotoxicity and detaching using low pH (6.0) PBS. mPAE showed no cytotoxicity and detached the human adipose derive stem cells (hADSCs) from the mPAE coated tissue culture plates (TCPs) by low pH (6.0) PBS. hADSCs cultured on and detached from mPAE coated TCPs showed similar cell adhesion and angiogenic paracrine factor secretion compared to hADSCs cultured on and detached from TCPs. Finally, hADSCs detached from mPAE coated TCPs showed similar angiogenesis and wound regeneration compared to hADSCs detached from TCPs. In conclusion, our mPAE might be utilized for enzyme free cell detachment system for future tissue engineering. |
| 저자 |
이태진, 박정환, 방석호
|
| 소속 |
성균관대 |
| 키워드 |
cell detachment; enzyme free; pH-sensitive polymer; skin regeneration
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| E-Mail |
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