| 초록 |
This study involved in the preparation and characterization of poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) and evaluation of their cellular uptake by using human gastric cancer cell line (SNU-638), as an in-vitro model. Drug-loaded PGD NPs were prepared by water/oil emulsion method and characterized by particle size analyzer, the average particle size ranges from 140 to 190 nm. Surface charge by zeta-potential indicates high electric charge with an average potential value.1 Surface morphology shows that the particles have moderate uniformity and narrow distribution. Cellular uptake efficiency of PGD NPs ranges from 56 to 64% after 2h incubation in SNU-638 cells. Confocal microscope clearly indicates that the NPs have been taken up by the cells.2 In-vitro cell cytotoxicity effect of drug-loaded NPs, when treated in SNU-368 didn’t show any cell toxicity for 48h. The PGD NPs showed significant advantages, with better effect, which have potential to use as drug carriers. |
