| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 가을 (10/06 ~ 10/07, 김대중 컨벤션센터) |
| 권호 |
36권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Polycaprolactone/Polyethylenimine Electrospun Nanofibers Mediated delivery of proliferation modulating factor for the treatment of Myocardial Infarction |
| 초록 |
we are presenting nanofibre mediated gene delivery for myocardial infarction. Branched polyethylenimine cross-linked via disulfide bonds (ssPEI) complexed with VEGF plasmid was immobilized on the nanofibers for the local expression of VEGF factor. We have studied whether the production of VEGF from myoblast cells adhering on the nanofibers has therapeutic potentials for myocardial infarction. Our results demonstrated that the delivery of therapeutic genes from biodegradable nanoparticles immobilized on the nanofiber represented minimal cytotoxicity to NIH/3T3 cells and H9C2 myoblasts than branched PEI 25kDa did. The results come from that this vector has high transgene expression efficiency. Surface-mediated delivery of DNA nanoparticles did not adversely affect cell growth and also facilitated the expression of transgene inside the cells.as a future work, we plan to deliver therapeutic microRNA through surface mediated delivery and evaluate its therapeutic effect in vitro and in vivo. |
| 저자 |
차혜련1, 안영근1, 김원종2, 조종수3, 박인규1
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| 소속 |
1전남대, 2포항대, 3서울대 |
| 키워드 |
Myocardial Infarction; Electrospun Nanofiber; ssPEI
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| E-Mail |
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