| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Metabolic rebalancing for optimization of 3-hydroxypropionic acid production pathway from glycerol in Escherichia coli |
| 초록 |
3-Hydroxypropionic acid (3-HP) can be biologically produced from glycerol by dehydration of glycerol to 3-hydroxypropionaldehyde (3-HPA) and oxidation of 3-HPA. In the pathway, imbalance between the two enzymatic reactions results in the accumulation of the toxic 3-HPA, which reduces 3-HP production. We used UTR engineering to maximize the activities of glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) for high conversion of glycerol to 3-HP. Thereafter, the activity of GDHt was precisely controlled to avoid the accumulation of 3-HPA by varying expression level. The optimally balanced E. coli HGL_DBK4 showed enhanced 3-HP titer (40.51 g/L) and productivity (1.35 g/L/h) compared with the parental strain. Especially, the yield on glycerol, 0.97 g 3-HP/g glycerol, in a fed-batch experiment, was the highest ever reported. Our study demonstrates that metabolic balancing is an effective strategy for the development of efficient microorganisms for bio-refinery production. |
| 저자 |
강채원1, 정규열1, 임현규1, 노명현1, 박성훈2
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| 소속 |
1포항공과대, 2울산과학기술원 |
| 키워드 |
3-Hydroxypropionic acid; Synthetic biology; Metabolic engineering; Balancing enzyme activity
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| E-Mail |
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