| 학회 | 한국화학공학회 |
| 학술대회 | 2009년 봄 (04/23 ~ 04/24, 광주 김대중컨벤션센터) |
| 권호 | 15권 1호, p.370 |
| 발표분야 | 생물화공 |
| 제목 | L-valine Production from Escherichia coli by Fed-batch Fermentation |
| 초록 | We have previously developed an Escherichia coli strain which is 100% genetically defined, producing high-yield L-valine in batch culture by targeted genome engineering combined with transcriptome analysis and gene knock-out simulation of the in silico genome-scale metabolic network. This engineered strain was able to produce 7.55 g/L L-valine with 20 g/L glucose, resulting in an impressively high yield of 0.378 g L-valine per g glucose. In this study, the remaining feedback inhibition was removed, and the development of a fed-batch fermentation process was performed. The results obtained here clearly demonstrate that the trade-off between L-valine and biomass formation should be optimized specifically towards L-valine production. Finally, the fed-batch culture of the further engineered strain allowed production of 32.3 g/L L-valine. To our knowledge, the L-valine concentration obtained here is the highest concentration ever reported in E. coli. [This work was supported by the Korean Systems Biology Project of the Ministry of Education, Science and Technology (M10309020000-03B5002-00000). Further supports by LG Chem Chair Professorship and Microsoft are appreciated] |
| 저자 | 박진환, 이광호, 김태용, 이종민, 이상엽 |
| 소속 | KAIST |
| 키워드 | L-valine; fed-batch; fermentation; Escherichia coli; systems biotechnology |
| 원문파일 | 초록 보기 |
