| 초록 |
In this study, we developed a human taste receptor protein, hTAS2R38-functionalized conducting polymer nanotube (CPNT)-field effect transistor (FET) as a nanobioelectronic tongue (nbe-tongue) that displayed human-like performance with high sensitivity and selectivity. Taster type (PAV) and non-taster type (AVI) hTAS2R38s were expressed in Escherichia coli (E. coli) at a high-level and immobilized on CPNT-FET sensor platform. Among the various tastants examined, PAV-CPNT-FET exclusively responded to target bitterness compounds, phenylthiocarbamide (PTC) and propylthiouracil (PROP) with high sensitivity at concentration as low as 1 fM. |
