| 초록 |
Superparamagnetic iron oxide nanoparticles with suitable biocompatible molecules have been widely used for in vivo applications such as magnetic resonance imaging contrast agent, tissue engineering, immunoassay, detoxification of biological fluids, hyperthermia, drug delivery and cell separation etc. Magnetite nanoparticles were prepared by coprecipitation of ferrous iron (Fe2+) and ferric iron (Fe3+) with NH4OH and dextran. End-funcionalized PEG were then coated onto the surface of the magnetite nanoparticles. Subsequently Homing peptide AP-1, which obtained by phage display technique was immobilized on the surface of the PEG-coated magnetites. The characterization of nanometer-sized magnetite nanoparticles were measured by XRD, FT-IR, TEM, DLS and VSM. Interaction of homing peptide-conjugated nanoparticles with bladder cancer cells were investkgated to examine intracellular uptake of the particles into target cells. |
