| 초록 |
Effective delivery of therapeutic proteins into the cells is an important issue for effective therapy of many diseases. In particular, cell-impermeable macromolecular proteins are a current research target, because they cannot be delivered without the aid of appropriate carrier systems. In this work, we develop the calcium carbonate-based hybrid nanoparticles for efficient intracellular protein delivery. An apoptotic protein, cytochrome C (Cyt C) loaded nanoparticles were prepared by poly(ethylene glycol)-b-polyaspartate) (PEG-b-PAsp) mediated in-situ CaCO3 mineralization in the presence of Cyt C. The pH dependent dissolution behavior of hybrid nanoparticles and the pH-dependent Cyt C release kinetics were examined. The efficacy of the intracellular delivery of Cyt C and the apoptotic functions for tumor cells were evaluated. |
