| 초록 |
Mesenchymal stem cells (MSCs) have been widely investigated for therapeutic applications due to the advantageous characteristics of facile isolation, prolonged transgene expression and hypo-immunogenicity. Here, we developed a new platform of supramolecular hyaluronate (HA) hydrogels encapsulating genetically engineered MSCs (eMSCs) expressing enhanced green fluorescence protein (EGFP) as a model system, mutant interleukin-12 for cancer therapy and hepatocyte growth factor for ischemic therapy. The simple mixing of CB[6]-HA, DAH-HA and drug-CB[6] in the presence of eMSCs resulted in in situ hydrogel formation encapsulating eMSCs. The eMSCs producing EGFP remained alive and emitted the fluorescence within CB[6]/DAH-HA hydrogels in mice for more than 60 days. The long-term expression of protein by eMSCs within the supramolecular hydrogels resulted in effective inhibition of tumor growth and the enhanced recovery of blood perfusion in hind limb ischemic model mice. |
