| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Temperature-sensitive nanogel using (lactic acid)10-pluronic-(lactic acid)10 and PLL-g-PEG as a gene carrier |
| 초록 | Tri-block copolymer pluronic is a temperature-sensitive and non-biodegradable polymer which self-assembles into micelle in aqueous solution at physiological temperature. Hydrophobic interaction, however, is weakened below the physiological temperature, e.g. 15 °C, to deconstruct the micelle structure. Novel polymeric temperature-sensitive nanogel was developed as a gene carrier using LA10-pluronic-LA10 as a temperature-sensitive and biodegradable moiety and positive-charged PLL-g-PEG as a gene complex moiety. Oil-in-water emulsion was used to construct core-shell structure having LA10-pluronic-LA10 inside the nanogel surrounded by PLL-g-PEG as the shell. 4-Nitrophenyl chloroformate-driven amine reaction was used to make chemical crosslink between the core and the shell. The nanogel size was 150 nm at 37 °C while the size reversibly increased at 15 °C using dynamic light scattering (DLS) and transmission electron microscope (TEM). Concentration of PLL-g-PEG and PEG-substitution rate of PLL-g-PEG showed great influence on the size of the nanogel. The nanogels having positive charge formed ionic complex with DNA which was verified by electrophoresis. The nanogel/DNA complex showed great rate of cellular uptake by cancer cells such as KB and PC3. Confocal microscopy was used to show cellular uptake of the nanogel/DNA complex by cancer cells such as KB and PC3. Also, the nanogel and nanogel/DNA complex inside cancer cells seems to have effect on maintaining cellular integrity during cold shock at 4 °C for a short time period (5 minutes). Increased size of the nanogel seems to be the major factor. |
| 저자 | 이유한, 박성영, 김선화, 박태관 |
| 소속 | 한국과학기술원 생명과학과 |
| 키워드 | temperature sensitive; nanogel; pluronic; poly(L-lysine) |
