| 초록 |
A plethora of important, chemically diverse natural products are derived from plants. In principle, plant cell culture offers an attractive option for producing many of these compounds. However, it is often not commercially viable because of difficulties associated with culturing DDCs on an industrial scale. To bypass the dedifferentiation step, we isolated and cultured innately undifferentiated CMCs. Using a combination of deep sequencing technologies, we identified marker genes and transcriptional programs consistent with a stem cell identity. This notion was further supported by the morphology of CMCs, their hypersensitivity to γ-irradiation and radiomimetic drugs and their ability to differentiate at high frequency. These cells may provide a cost-effective and environmentally friendly platform for sustainable production of a variety of important plant natural products in such as cosmetics industry. |
