| 초록 |
Cell membranes are composed of various kinds of lipids and cholesterols. Mixing and demixing of such different lipids (e.g. lipid rafts and/or micron-sized domains) in cell memebranes have been known to be crucial for many cellular processes, but it is not well understood. We have recentely developed a new technique, called FRAM (Fluorescence Recovery After Merging a phospholipid covered droplet), to measure diffusion of lipid monolayers and to directly visualize different lipid monolayers being mixed. Using this technique, we found that oxidized-unsaturated phospholipids are phase-separated from cholesterols, and even demix completely depending on the degree of oxidation. |
