| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Functional expression of phospholipase A1 from Serratia sp. MK1 using Escherichia coli system |
| 초록 |
Phospholipase A1 (EC 3.1.1.32) is an enzyme that hydrolyzes phospholipid into a fatty acid and a lysophosphoslipid and has potential industrial applications in such fields as bioenergy, food, and pharmaceutical industry. Phospholipase has lipolytic activity, however, causes inhibition of cell growth and phospholipase hinders its production in the conventional cell-based expression methods. Because it does not require membrane integrity for protein synthesis, cell-free protein synthesis system using cell lysates can offer an alternative route. In this study, we attempted to express the phospholipase A1 gene cloned from the chromosomal DNA of Serratia sp. MK1 either in E.coli cells or a cell-free synthesis system derived from the lysate of the E.coli cells. From the same amount of cellular protein, cell-free protein synthesis produced over a 1000-fold higher titer of functional phospholipase A1 than standard recombinant techniques using whole E.coli cells. |
| 저자 |
임혜진1, 박유진1, 장연재1, Devi Casi1, Christy Catherine1, 이경호1, Tong Yi1, 이종두1, 박지현2, 이소정2, 오준영2, 송재광2, 김동명1
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| 소속 |
1충남대, 2한국화학(연) |
| 키워드 |
Phospholipase; Cell-free protein synthesis; Escherichia coli
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| E-Mail |
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