| 초록 |
Controlling release of growth factors from hydrogels has been challenging because encapsulated proteins often show initial burst release profiles due to fast diffusion process. Heparin enhances a half-life and activity of bFGF, also, protects bFGF from inactivation by acid and heat. In this study, hydrogel (22%, w/w) composed of di-acrylated Pluronic F127 and glycidyl methaacrylated chitooligosaccharide (COS) (0, 20, and 50%, w/w) was prepared in an aim to control release of bFGF. A photo-initiator, Irgacure2959 (0.01%, w/w), was added to the polymer mixture. In order to measure release profile of bFGF from the hydrogels in vitro, the released bFGFs were measured by ELISA. Due to the electrostatic interaction between COS and heparin, when a content of COS was 50%, an initial burst release of bFGF was reduced 2.4-fold and 1.4-fold compared with those of 0 and 20%, respectively. The chemically-crosslinked hydrogels containing bFGF/heparin can be a candidate for drug delivery system. |
