| 학회 |
한국화학공학회 |
| 학술대회 |
2005년 봄 (04/22 ~ 04/23, 여수대학교) |
| 권호 |
11권 1호, p.362 |
| 발표분야 |
생물화공 |
| 제목 |
Fed-batch fermentation of recombinant Escherichia coli harboring the ddsA gene and the dxs gene improved production of coenzyme Q10 |
| 초록 |
Recently, coenzyme Q10 has been interested with respect to its physiological functions such as pro-oxidant and anti-oxidant activity. Types coenzyme Q in organisms are determined by the availability of the polyprenyl diphosphate which is catalyzed by polyprenyl diphosphate synthase. As Escherichia coli has endogenous octaprenyl diphosphate synthase, it can produce coenzyme Q8 instead of coenzyme Q10. In order to produce coenzyme Q10 in E. coli, the ddsA gene encoding decaprenyl diphosphate synthase derived from Gluconobacter suboxydans was cloned and expressed a constitutively. The dxs gene was coexpressed with the ddsA gene in order to increase the specific content of coenzymeQ10. As production of coenzyme Q10 is dependent on cell growth, fed-batch fermentation was carried out to obtain high cell density and high concentration of coenzyme Q10. A significant increase of dry cell mass in the fed-batch fermentation allowed coenzyme Q10 concentration of 46.1mg/l, corresponding to a 27-fold increase compared with the batch fermentation. |
| 저자 |
김수정1, 최진호1, 민원기1, 김성건1, 하석진2, 김상용2, 서진호1
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| 소속 |
1서울대, 2바이오앤진 |
| 키워드 |
Coenzyme Q10; recombinant E. coli; ddsA; dxs; metabolic engineering; fed-batch fermentation
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| E-Mail |
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| 원문파일 |
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