화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU))
권호 26권 1호
발표분야 포스터-생물공학
제목 Lactobionic acid production​ by genetically engineered Pseudomonas taetrolens using residual food dried substance as a culture medium.​
초록 Using residual food dried substance (RFDS) derived from food waste (FW) by the action of home-service food drying machine as a culture nutrient, we tried to produce lactobionic acid (LBA) from lactose by cultivating an LBA-producing bacterium, Pseudomonas taetrolens. We prepared culture medium for the LBA production by grinding RFDS finely and dissolving this fine powder into deionized water. To improve the LBA production, we supplemented three hydrolytic enzymes including amylase, protease, and lipase separately or simultaneously into the culture medium during the cultivation of P. taetrolens strain. The addition of amylase was most effective to increase the LBA production. We scaled up the LBA production using a 5-L fermenter. From the above results, we demonstrated that the RFDS from FW could be used as the culture nutrient for the production of a value-added chemical.
저자 조채연, 한소연, 엄경태
소속 한국화학(연)
키워드 Food waste; residual food dried substance; culture medium; lactobionic acid
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