초록 |
Using residual food dried substance (RFDS) derived from food waste (FW) by the action of home-service food drying machine as a culture nutrient, we tried to produce lactobionic acid (LBA) from lactose by cultivating an LBA-producing bacterium, Pseudomonas taetrolens. We prepared culture medium for the LBA production by grinding RFDS finely and dissolving this fine powder into deionized water. To improve the LBA production, we supplemented three hydrolytic enzymes including amylase, protease, and lipase separately or simultaneously into the culture medium during the cultivation of P. taetrolens strain. The addition of amylase was most effective to increase the LBA production. We scaled up the LBA production using a 5-L fermenter. From the above results, we demonstrated that the RFDS from FW could be used as the culture nutrient for the production of a value-added chemical. |