| 초록 |
Branched polyethylenimine was a high gene transfection agent. However, bPEI utilization as gene carrier was limited because of high cytotoxicity. To overcome these obstacle, chitosan was introduced to bPEI by CDI as coupling agent. The chemical structure of chitosan-g-bPEI was analyzed by 1H-NMR. The particle size, zeta potential and morphological property of binary and ternary complexes were confirmed by DLS and TEM. Moreover, transfection efficiency and cellular uptake showed that gene expression and internalization of ternary complexes were enhanced more than binary complexes. The targeting effect of ternary complexes was confirmed by competition assay in CD44-overexpressed HCT116 cell line. These results suggest that ternary complex is superb gene carrier with targeting effect against CD44-receptor positive cancer cell line. (No. NRF-2016H1D5A1910499). |
