화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO))
권호 23권 1호
발표분야 생체재료_포스터
제목 Tumor-targeted delivery of CRISPR plasmid using HA-functionalized carbon dots
초록 Gene therapy is a promising technology for cancer treatment due to the versatility and specificity of the drug. The CRISPR/Cas9 system has been attractive as a gene therapy since the target gene can be reprogrammed with high efficiency and selectivity. A non-viral method can effectively deliver the cargo to the target site without the problem from the viral method. Herein, polyethyleneimine carbon dots (PEI Cdots) were used to package efficiently the CRISPR plasmid and delivery them into cancer cells. A CRISPR plasmid which expressing Cas9 and sgRNA was prepared, complexed with PEI Cdots and functionalized with hyaluronic acid (HA) to both stabilize the complex and specifically target CD44 expressed on tumor cells. The HA-functionalized PEI Cdot/plasmid complex was characterized by dynamic light scattering and zeta potential measurements. Delivery of the complex into tumor cells was investigated and observing cellular uptake and knockout of the reporter gene by confocal microscopy.
저자 이주희, 정현정
소속 한국과학기술원
키워드 gene therapy; nanomaterial
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