| 초록 |
Self-assembled structure of monodisperse colloidal particles can be used for various applications such as photonic crystals and chemical sensing materials. Typical building blocks of self-assembly are polystyrene or polymethylmethacrylate latex spheres and silica suspension with narrow size distribution. Several methods have been developed to prepare self-assembled structure of these building blocks such as colloidal crystallization of microspheres in aqueous droplet. The drawback of this method is that the crystallization process is difficult to control the particle periodicity and interparticle distances. The utilization of DNA as molecular linker can allow us to overcome these drawbacks. In 1996, Mirkin group has succeeded to prepare the controlled assembly of gold nanoparticles by using the adsorption of thiol-terminated complementary oligonucleotides as linker molecules. They used the hybridization reaction of oligonucleotides with complementary base sequences to assemble gold colloid to macroscopic aggregate. However, only few articles presented the preparation of colloidal assembly of monodisperse polymeric microspheres by sequence specific hybridization of oligonucleotides. Here we describe a method for assembling monodisperse polystyrene microspheres by using oligonucleotide as linker molecule. Polystyrene building blocks were synthesized by soapless emulsion polymerization, and the hybridization of these building blocks was verified by combining two different sized microspheres adsorbed with oligonucleotides. |
