| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 |
23권 2호, p.1703 |
| 발표분야 |
생물화공 |
| 제목 |
Metabolic Engineering of Corynebacterium glutamicum for Large-scale production of L-Arginine |
| 초록 |
Industrial amino acids powerhouse Corynebacterium glutamicum was metabolically engineered for L-arginine production. First, random mutagenesis was performed on C. glutamicum to increase tolerance to L-arginine. By downregulating the pgi gene and overexpressing the opcA, pgl, tal, tkt, and zwf genes, PPP flux was strengthened. In addition, argF,carAB and argGH gene expression levels were optimized to convert L-ornithine to L-citrulline effectively.Fed-batch fermentation of the final strain in a 1,500 L bioreactor resulted in 81 g/L of L-arginine production. These approaches will be useful in developing Corynebacteria for the production of arginine and its derivatives. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning through the National Research Foundation of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557).] |
| 저자 |
한태희1, 조재성1, 박석현1, 김현욱2, 김태용2, 박준석3, 김석수3, 이상엽2
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| 소속 |
1한국과학기술원, 2한국과학기술원 / BioInformatics Research Center, 3Daesang corporation research center |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
