| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 가을 (10/07 ~ 10/08, 대구 EXCO) |
| 권호 |
35권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Cooperative binding of c-reactive protein to molecularly imprinted phosphocholine monolayer |
| 초록 |
We report molecular imprinting of c-reactive protein(CRP) on a gold surface and the binding characteristics of the imprinted monolayer(MIM). 12-Mercaptododecyl phosphocholine (PC12SH) was synthesized and five molecules of PC12SH were reacted with one CRP molecule. The CRP-PC12SH complex was allowed to adsorb onto a gold surface, preserving the ligand arrangement of pentameric CRP-specific binding sites. The blocking was performed by BSA or 2-mercaproethanol(ME). The KA was calculated by Langmuir isothermal equation, using SPR measurement. In case of BSA-blocked substrate, the KA of MIM(2.65X109/M) was approximately 8.6 folds higher than that of non-imprinted monolayer(NIM). With ME-blocked substrate, the KA of MIM was obtained as 6.08X107/M. In Hill polt analysis, the MIM showed non-homogeneous binding characteristics on dependent of CRP concentration, which showed increased cooperativity in CRP binding at high CRP concentration. However, NIM showed homogeneous binding characteristics. |
| 저자 |
김은주, 김현철, 이세근, 이성준, 백철수, 박종원, 정상원
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| 소속 |
대구경북과학기술원 |
| 키워드 |
c-reactive protein; 2D molecular imprinting; synthetic antibody sensor; cooperative binding
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| E-Mail |
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