| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Efficient conversion of acetate to 3-hydroxypropionic acid by engineered Escherichia coli |
| 초록 |
Acetate, which is an abundant carbon source, is a potential feedstock for microbial processes that produce diverse value-added chemicals. In this study, we produced 3-hydroxypropionic acid from acetate with engineered Escherichia coli. For the efficient conversion of acetate to 3-HP, we initially introduced heterologous mcr gene from Chloroflexus aurantiacus. Then, the acetate assimilating pathway and glyoxylate shunt pathway were activated by overexpressing acs gene and deleting iclR gene. Because a key precursor malonyl-CoA is also consumed for fatty acid synthesis, we decreased carbon flux to fatty acid synthesis by adding cerulenin. Subsequently, we found that inhibiting fatty acid synthesis dramatically improved 3-HP production (3.00 g/L of 3-HP from 8.98 g/L of acetate). The results indicated that acetate can be used as a promising carbon source for microbial processes and that 3-HP can be produced from acetate with a high yield (44.6% of the theoretical maximum yield). |
| 저자 |
차상학1, 이지훈1, 강채원1, 이건민1, 임현규2, 정규열1
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| 소속 |
1POSTECH, 2Univ. of California San Diego |
| 키워드 |
Metabolic engineering; Synthetic biology; 3-hydroxypropionic acid; Microbial production; Fatty acid synthesis; Acetate
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| E-Mail |
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