| 학회 |
한국고분자학회 |
| 학술대회 |
2006년 봄 (04/06 ~ 04/07, 일산킨텍스) |
| 권호 |
31권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Evaluation of various sizes of PLGA microspheres to be used in an injectable system for tissue engineered intervertebral disc |
| 초록 |
To overcome limited success of surgical IVD treatment, tissue engineering offers alternative techniques. We assessed the cell growth behaviors with PLGA microspheres. We fabricated the various sizes of PLGA microspheres using O/W method. Morphology of PLGA microspheres were characterized by SEM. NP cells were used of attachment, proliferation, and gene expression within the various sizes of PLGA microspheres. Cell morphology was studied by SEM. Cell proliferation was analyzed using MTT. We observed specific gene expression of NP cell in RT-PCR. SEM showed that cells could bridge between microspheres. MTT revealed that the cells proliferated the PLGA microspheres in the range with 250 μm. In RT-PCR, there was not a significant difference between the levels of specific NP cell gene expression. The PLGA microshperes could create an injectable system for tissue engineered intervertebral disc. This research was supported by Musculoskeletal Bioorgan Center (0405-BO01-0204-0006) by KMOHW. |
| 저자 |
최방실1, 고종태1, 김순희1, 하현정1, 백미옥1, 이종문1, 김문석2, 이해방2, 강길선1
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| 소속 |
1전북대, 2한국화학(연) |
| 키워드 |
IVD; NP cell; PLGA microsphere
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| E-Mail |
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