| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 가을 (11/04 ~ 11/06, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
19권 2호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
The amine-lipid based lipomers for mRNA delivery into MEF cells |
| 초록 |
Delivery of messenger RNAs (mRNAs) has drawn much attention since it show great advantages over conventional plasmid DNA (pDNA) delivery. We have developed new amine-lipid based lipomers for efficient delivery of mRNA to mouse embryonic fibroblast (MEF) cells. Resulting lipomers show facile self-assembly with negatively charged mRNA through electrostatic interaction. A library of eight different amine-lipid based lipomers has been screened by in vitro gene transfection. As a result, amine-epoxydodecane (102-C10) show excellent delivery of mRNA and thereby express high Green Fluorescence Protein (GFP) expression in MEF cells. In addition, the viability of tansfected cells is well maintained using a 102-C10 lipomer. These results indicate that amine-lipid based lipomers have significant potential as a promising mRNA delivery carriers. |
| 저자 |
이혁진, 정한샘
|
| 소속 |
이화여자대 |
| 키워드 |
lipid based lipomers; mRNA delivery; gene expression
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| E-Mail |
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