| 초록 |
The extracellular matrix (ECM) can influence on regulation of cell functions such as cell adhesion, migration, proliferation, and differentiation. The cell derived ECM (CD-ECM) is a useful in vitro model for studying the comprehensive functions of CD-ECM because it maintains a native-like structure and composition. In this study, the CD-ECM is obtained and a test is carried out to determine the effectiveness of several combinations of decellularized methods. We investigated the effect of osteoblasts re-seeded onto normal osteoblast ECM under the growth medium ECM (GM-ECM) and the osteogenic differentiation medium (OD-ECM). The osteoblasts were then cultured for 1, 2, and 4 weeks in a growth medium or differentiation medium. After each culture period, the osteogenic differentiation of the osteoblasts re-seeded on the OD-ECMs was enhanced osteogenic differentiation. The OD-ECM-4W group showed more effective osteogenic differentiation than GM-ECM and OD-ECM-2W. |
