| 학회 |
한국화학공학회 |
| 학술대회 |
2009년 봄 (04/23 ~ 04/24, 광주 김대중컨벤션센터) |
| 권호 |
15권 1호, p.378 |
| 발표분야 |
생물화공 |
| 제목 |
Overproduction of trehalose by recombinant Escherichia coli in the presence of validamycin A and high osmolarity |
| 초록 |
In this presentation, we explored the trehalose production in E.coli by over-expressing trehalose biosynthetic operon (otsBA) encoding trehalose-6-phosphate phosphatase and trehalose-6-phosphate synthase as well as inhibiting the action of trehalase in the cells. These genes were amplified by PCR, sequenced and cloned into pTrc99A vector. The recombinant DNA was introduced into E.coli cells. The productivity of trehalose was investigated in the fermentation of recombinant E.coli cells in M9 media with or without high osmolarity or validamycin A. In vitro enzyme assays using cell-free extract were also performed to verify the action of trehalase when trehalose degradation occurred. It was found that trehalase activity in the cells was the highest under the high osmotic pressure. Addition of trehalase inhibitor, validamycin A into medium inhibited the trehalase action in the cells and thus significant enhancement of trehalose production in the recombinant cells was observed under the osmotic pressure. |
| 저자 |
He Li1, 김창준1, 김성배1, 장용근2, 홍순광3, 서양곤1
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| 소속 |
1경상대, 2한국과학기술원 생명화학공학과, 3명지대 |
| 키워드 |
Trehalose; Overexpression; validamycin A; trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase; recombinant Escherichia coli
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| E-Mail |
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| 원문파일 |
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