| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Development of a fed-batch culture process of recombinant Escherichia coli for the production of urate oxidase incorporated with unnatural amino acid |
| 초록 |
In this study, a fed-batch culture process of recombinant Escherichia coli for the production of recombinant urate oxidase incorporated with unnatural amino acid, 4-azido-phenylalanine(AzF). Firstly, through a series of flask cultures of 10 types of recombinant E. coli, which were transformed with two plasmids of pEVOL_pAzF and pQE80_UoxR for the expression of urate oxidase incorporated with Azf (UO_Azf), recombinant E. coli Top10 was selected as the best strain for the production of UO_Azf. Using a recombinant E. coli Top10 strain, a series of fed-batch cultures were conducted by varying several variables such as the IPTG induction time, the amount of glucose and complex nitrogen sources fed per feeding and the Azf concentration. As a result, it was confirmed that the specific activity of UO_Azf increased to a maximum of 41.14 U/mg when the culture was operated at the best condition. |
| 저자 |
정용배1, 송지수1, 김자경1, 심명섭1, 김겸민1, 김현우2, 최종일1, 김태완1
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| 소속 |
1전남대, 2(주)프로앱텍 |
| 키워드 |
urate oxidase; unnatural amino acid; E. coli
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| E-Mail |
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