| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
A plug-in repressor library for precise rebalancing of metabolic flux in Escherichia coli. |
| 초록 |
Although advances in synthetic biology have achieved the exploitation of a number of genetic elements for precise flux control, their use requires expensive inducers or needs complex and time-consuming processes to design and optimize adequate regulator components, case-by-case. So, we devised a plug-in phlF repressor library for target-specific flux control, where its expression level was diversified using degenerate 5’ untranslated region (5’ UTR) sequences. After validation, it was applied to enhance the production of 3-hydroxypropionic acid (3-HP) and lycopene production. Consequently, we successfully achieved optimal carbon fluxes around the precursor nodes and the most optimized strains produced 1.08 g/L of 3-HP and 10.6 mg/L of lycopene, 5.5-fold and 1.9-fold respectively compared to parental strains. These results indicate that plug-in phlF library is a powerful strategy for efficient production of value-added chemicals by precisely regulating carbon flux in E. coli. |
| 저자 |
이지연1, 차상학1, 이지훈1, 임현규2, 노명현1, 강채원1, 정규열1
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| 소속 |
1포항공과대, 2UCSD |
| 키워드 |
Metabolic engineering; carbon flux control; 5' UTR; repressor; 3-hydroxypropionic acid; lycopene
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| E-Mail |
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