| 초록 |
Many studies have been reported on polymeric particulate systems for controlled delivery of proteins. But, these systems using biodegradable polymers raised problems such as low stability and encapsulation efficiency of proteins due to water/organic emulsification. Our group has reported nanoaggregates (NAs) of Pluronic-grafted polysaccharides for controlled release of drugs. In this study, Protein-loaded NAs was prepared and investigated the intracellular uptake and protein release of the NAs. The size and surface morphology of NAs were analyzed by DLS and TEM. The surface charge of prepared NAs was determined by Zeta potential. The protein release from the NAs was monitored to investigate the release behaviors. Structural stability of proteins during NA formation was confirmed by circular dichroim and fluorescence spectroscopy. In cellular experiments, cytoplasmic uptake of protein-loaded NAs was observed by confocal microscope and quantified by fluorescence-activated cell sorter. |
