| 초록 |
Monolithic molecularly imprinted column was prepared by an in-situ therm-initiated copolymerization using theophylline, methacrylic acid and ethylene glycol dimethacrylate with a mixture of toluene-dodecanol as a porogenic solvent. Effects of the polymerization mixture composition and polymerization condition, template molecule, mobile phase composition, flow rate and temperature on the retention and separations were investigated. Due to the large throughput pores in the monolithic column, low backpressure was observed during the separation process and separation factor of 3.5 was obtained at 1.0 ml/min. This type of molecularly imprinting column can be prepared by a simple, one-step, in-situ polymerization process without the tedious procedures of grinding, sieving, and column packing. The method is very simple compared with the conventional procedure and its macroporous structure has excellent separation properties. |
