| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Hyaluronic acid/calcium phosphate hybrid gene delivery system enhanced transfection efficiency of human mesenchymal stem cells. |
| 초록 |
Human mesenchymal stem cells (hMSCs) have shown enormous potential in regenerative medicine and tissue engineering. However, current use of hMSCs in clinics is still limited since there is no appropriate way to control their behaviors in vivo such as differentiation to a desired cell type. Genetic modification of hMSCs may provide an opportunity of controlling behaviors of the cells in an active manner. One of the major huddles for genetic manipulation of hMSCs would be lack of efficient and safe gene delivery system. Herein, an organic-inorganic hybrid gene delivery system based on calcium phosphate (CAP) nanoparticles stabilized with catechol-derivatized hyaluronic acid (dopa-HA) conjugate. The dopa-HA/CAP nanoparticles showed significantly higher transfection in hMSCs than PEI 25kDa. In addition, delivery of BMP2 plasmid DNA with the dopa-HA/CAP formulation achieved desired level of osteogenic differentiation of hMSCs. In this study, we introduced a new organic-inorganic hybrid gene delivery system for enhanced transfection of hMSCs. Taking advantage of specific interactions between HA and CD44+ bone marrow-derived hMSCs, dopa-HA/CAP/pDNA-BMP2 formulation achieved desired level of BMP2 gene expression, resulting in successful osteogenic differentiation of hMSCs. Considering its biocompatibility and biodegradability, the CAP nanoparticle stabilized by dopa-HA can be potentially used for genetic manipulation of hMSCs in clinical settings. |
| 저자 |
이정은1, 이민상1, 조용우2, 조현철3, 정지훈1
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| 소속 |
1성균관대, 2한양대, 3서울대 |
| 키워드 |
calcium phosphate; stem cell; hyaluronic acid
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| E-Mail |
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