| 초록 |
Human mesenchymal stem cells (hMSCs) have shown enormous potential in regenerative medicine and tissue engineering. One of the possible approaches to direct differentiation of hMSCs would be transferring extraneous genes to guide the cells’ differentiation to desired fate. However, differentiation of hMSCs to desired cells is often difficult to control especially in vivo situation. Genetic modification of hMSCs may provide an opportunity of controlling behaviors of the cells in an active manner. One of the major huddles for genetic manipulation of hMSCs would be lack of efficient and safe gene delivery system. Herein, an organic-inorganic hybrid gene delivery system based on calcium phosphate (CAP) nanoparticles stabilized with catechol-derivatized hyaluronic acid (dopa-HA) conjugate. Taking advantage of specific interactions between HA and CD44+ bone marrow-derived hMSCs, dopa-HA/CAP nanoparticles showed significantly higher transfection in hMSCs than PEI 25kDa, and delivery of BMP2 pDNA with the dopa-HA/CAP formulation achieved desired level of osteogenic differentiation of hMSCs. So it can be potentially used for genetic manipulation of hMSCs in clinical settings. |
