| 학회 |
한국공업화학회 |
| 학술대회 |
2010년 봄 (05/13 ~ 05/14, BEXCO(부산)) |
| 권호 |
14권 1호 |
| 발표분야 |
정밀화학 |
| 제목 |
A new chemical tool for the identification of O-GlcNAc porteome, O-GlcNAz-modified proteins |
| 초록 |
We engineered a phosphine reagent by installing an easily cleavable disulfide linker between the phosphine skeleton and a biologically active tag such as His-tag or biotin. Using this reagent, O-GlcNAc-modified proteins (or peptides) are distinguished from proteins that are not O-GlcNAc-modified.In this strategy, tagged O-GlcNAc-modified proteins bind to functionalized magnetic nanoparticles allowing the O-GlcNAc bound nanoparticles to be easily isolated from the whole cell extract.Then, O-GlcNAz-modified proteins are easily released from the nanoparticle by breaking disulfide linkage of the engineered phosphine reagent.This new chemical tool will accelerate the identification of O-GlcNAc modified proteins and the site of modification. |
| 저자 |
김은주 |
| 소속 |
대구대 |
| 키워드 |
O-GlcNAc; magnatic nanoparticles; disulfide linker
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| E-Mail |
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